北京大学学报（医学版）2011，Vol.43Issue(3)：348-351,4.DOI:10.3969/j.issn.1671-167X.2011.03.007
雌马酚对二甲基苯蒽诱导的去势大鼠乳腺肿瘤的影响
Effect of equol on breast tumors induced by 7, 12-dimethylbenz (α) anthracene in ovariectominzed rats
摘要
Abstract
Objective : To study the effect of equol on the incidence of breast tumors induced by 7 , 12dimethylbenz( a) anthracene ( DMBA) in ovariectomized rats. Methods: In the study, 48 female ovariectomized SD rats were randomly divided into 6 groups according to their weights: blank control group, fed on an AIN-93G diet; model group, fed on an AIN-93G diet; estrogen control group, fed on an AIN93G diet supplemented with 0. 25 mg/kg diethylstilbestrol; and low, medium and high doses of equol groups , fed on an AIN-93G diet supplemented with 100 , 200 and 400 mg/kg diet of equol, respectively. The animals were palpated to examine the breast tumor, body weight and dietary intake of each rat per week. The data were recorded. At the end of the experiment, the incidence of breast tumor was calculated and the sera were collected for the detection of SOD, MDA and 8-OHdG levels. The expression of Nrf2 in liver was assessed by Western-blotting. Results : Equol decreased the rats' body weights , dietary intakes, breast tumor incidence and MDA levels, but had no effect on the levels of SOD and 8-OHdG in the sera. The expression of Nrf2 was up-regulated dose-dependently in equol groups. Conclusion : Supplementation of equol could decrease the incidence of breast tumor in ovariectominzed rats. This effect may result from the reduction of body weight, intake of diet,MDA level in the sera and up-regulation of expression of Nrf2. The effect of equol may be influenced by many factors, such as the structure of equol, which should be further studied.关键词
雌马酚/乳腺肿瘤/抗氧化剂/大鼠Key words
Equol/ Breast neoplasms/ Antioxidants/ Rats分类
医药卫生引用本文复制引用
赵晓慧,胡建伟,张玉梅,王培玉..雌马酚对二甲基苯蒽诱导的去势大鼠乳腺肿瘤的影响[J].北京大学学报（医学版）,2011,43(3):348-351,4.基金项目
国家自然科学基金(30671759,30872114,30872115)资助 （30671759,30872114,30872115）
