中国组织工程研究与临床康复2011，Vol.15Issue(20)：3728-3732,5.DOI:10.3969/j.issn.1673-8225.2011.20.028
三七总皂苷干预股骨头缺血性坏死兔成骨细胞护骨素基因的表达
Total saponins of Panax notoginseng effects on mRNA expression of osteoprotegerin in osteoblasts from rabbits with avascular necrosis of the femoral head
摘要
Abstract
BACKGROUND: Previous studies have demonstrated that total saponins of Panax notoginseng (PNS) can inhibit the ethanol-induced adipogenic differentiation of rabbit bone marrow stromal cells (BMSCs).OBJECTIVE: To observe PNS effects on osteoblast proliferation and mRNA expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL).METHODS: Rabbit models of alcohol-induced avascular necrosis of the femoral head were divided randomly into saline group, compound bone peptide group, PNS group. Rabbit osteoblasts were extracted for measurement of OPG and RANK mRNA expressions and hybridizing signal intensity.RESULTS AND CONCLUSION: In the PNS group, alkaline phosphatase activity, calcium nodule count, OPG and RANK mRNA expressions and their ratio were significantly higher than those in the saline group (P ＜ 0.01), and similar to those in the compound bone peptide group. The experiment confirmed that PNS can promote the proliferation and differentiation of rabbit osteoblasts, and can improve the OPG mRNA relative expression in osteoblasts so as to inhibit RANKL mRNA expression.关键词
三七总皂苷/乙醇中毒/股骨头坏死/成骨细胞/骨保护素分类
医药卫生引用本文复制引用
王大伟,史宝明,李小峰,张爽,莫坚,顾国龙,林宝举,马开好,惠宁军..三七总皂苷干预股骨头缺血性坏死兔成骨细胞护骨素基因的表达[J].中国组织工程研究与临床康复,2011,15(20):3728-3732,5.基金项目
广西卫生厅重点科研课题(重200853),三七总皂苷对兔股骨头缺血性坏死成骨细胞护骨素基因表达的影响. （重200853）
