高等学校化学学报2011，Vol.32Issue(5)：1221-1224,4.
罗丹明B与基因共负载纳米粒子的制备与性能
Construction of Rhodamine B and Gene Co-delivery Nanoparticles
摘要
Abstract
The hydrophobic fluorescent dye rhodamine B was chosen as a model drug. PEI-g-cholesterol amphiphilic polymer was synthesized as drug carrier. By selecting proper conditions, rhodamine B and gene codelivery nanoparticles were successfully constructed via supramolecular assembly. The co-delivery particles with diameter about 154 nm and ξ-potential about 33.7 mV could effectively condense DNA. The in vitro cell culture experiments indicated that the positive co-delivery nanoparticles were easily uptaked by HEK293 cells and showed effective transfection efficiency.关键词
非病毒基因载体/超分子组装/药物/基因共负载Key words
Non-viral gene vector/ Supramolecular assembly/ Drug and gene co-delivery分类
化学化工引用本文复制引用
王幽香,沈潇波,徐志学,胡巧玲..罗丹明B与基因共负载纳米粒子的制备与性能[J].高等学校化学学报,2011,32(5):1221-1224,4.基金项目
国家自然科学基金(批准号:50873089,21074110)、浙江省自然科学基金(批准号:Y4100101)、浙江省科技计划项目(批准号:2010C31025)和中央高校基本科研业务费专项基金(批准号:2011QNA4025)资助. （批准号:50873089,21074110）
