中国组织工程研究与临床康复2011，Vol.15Issue(20)：3785-3788,4.DOI:10.3969/j.issn.1673-8225.2011.20.043
Fas及FasL和Caspase-3在青蒿琥酯诱导人胚肺成纤维细胞凋亡中的表达
Role of Fas, FasL and Caspase-3 in artesunate-induced apoptosis of human embryonic lung fibroblasts
摘要
Abstract
BACKGROUND: Artesunate can relieve pulmonary fibrosis, but its mechanisms are rarely reported.OBJECTIVE: To explore the effect of artesunate on apoptosis of HFL-I cells and the role of Fas, FasL and Caspase-3 in the artesunate-mediated apoptosis of HFL-I cells. METHODS: Cell counting kit-8 (CCK-8) assay was used to determine the effects of artesunate at 1, 10, 100 mg/L on the growth of HFL-I cells in vitro. Apoptosis ratio was examined by flow cytometry (FCM). The mRNA level of Fas, FasL and Caspase-3, were assessed by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS AND CONCLUSION: Artesunate had a significantly inhibitory effect on the proliferation of HFL-I cells in a dose-dependent manner in vitro. The apoptosis rate of HFL-I cells was significantly increased in the artesunate-treated group compared with the control group (P < 0.05 or P < 0.01). The mRNA levels of Fas, FasL and Caspase-3 were significantly higher in the artesunate-treated group than the control group (P < 0.05). The findings of this study demonstrate that artesunate can exert a marked anti-pulmonary fibrosis effect by up-regulating mRNA levels of Fas, FasL and Caspase-3, which can induce the growth inhibition and apoptosis in HFL-I cells.关键词
青蒿琥酯/人胚肺成纤维细胞/凋亡/增殖/组织工程分类
医药卫生引用本文复制引用
王昌明,黎洪秀,张孝飞..Fas及FasL和Caspase-3在青蒿琥酯诱导人胚肺成纤维细胞凋亡中的表达[J].中国组织工程研究与临床康复,2011,15(20):3785-3788,4.基金项目
Supported by:Science Research and Technology Development Plan of Guangxi Province,No.10124008-4 课题受广西科学研究与技术开发计划课题资助(桂科攻10124008-4).课题名称:青蒿琥酯对肺成纤维细胞增殖影响的分子生物学机制研究. （桂科攻10124008-4）
