中国组织工程研究与临床康复2011，Vol.15Issue(20)：3789-3792,4.DOI:10.3969/j.issn.1673-8225.2011.20.044
周期性张应力诱导成纤维细胞凋亡中p38MAPK信号通路的作用
Effects of p38MAPK signaling pathway on cyclic tensile stress-induced fibroblast apoptosis
摘要
Abstract
BACKGROUND: When the teeth affected abnormal biting force, tooth absorption and periodontium would be greatly damaged. OBJECTIVE: To study whether periodontal membrane fibroblast affected apoptosis following cyclic tensile stress stimulation and whether p38MAPK signaling pathway participated in apoptosis. METHODS: Fibroblasts at passages from 4 to 7 were randomly assigned to control, loading and SB203580 groups after synchronization. In the loading and SB203580 groups, 12% strain was applied at a loading frequency of 6 cycles per minute, i.e. 5 seconds for tension and 5 seconds for relaxation. In the SB203580 group, cells were treated with 20 mmol/L p38MAPK inhibitor SB203580 at 1 hour before loading. At 6, 12 and 24 hours after loading, cells from each group were harvested, and cell apoptosis was detected using a flow cytometry. Expression of bax mRNA was determined using reverse transcription-polymerase chain reaction. RESULTS AND CONCLUSION: Compared with the control group, apoptotic rate of fibroblasts and bax mRNA expression were increased after loading (P < 0.05), and enhanced over time, and peaked at 12 hour following loading, and then decreased gradually. Compared with the loading group, cell apoptosis was reduced at corresponding time points in the SB203580 group (P < 0.05), and bax mRNA expression was diminished. These results indicated that cells affected apoptosis after mechanics stimulation, and mitogen activated protein kinase p38MAPK signaling pathway participates in the process of apoptosis.关键词
丝裂原活化蛋白激酶/周期性张应力/凋亡/成纤维细胞分类
医药卫生引用本文复制引用
仇静,张广耘,田臻,张月,于江波,袁晓..周期性张应力诱导成纤维细胞凋亡中p38MAPK信号通路的作用[J].中国组织工程研究与临床康复,2011,15(20):3789-3792,4.基金项目
Supported by:the National Natural Science Foundation of China,No.30871426.国家自然科学基金(30871426),课题名称:p38MAPK信号通路在周期性张应力诱导的成纤维细胞凋亡中的作用. （30871426）
