中国组织工程研究与临床康复2011，Vol.15Issue(23)：4185-4188,4.DOI:10.3969/j.issn.1673-8225.2011.23.002
大鼠骨髓间充质干细胞体外向成骨细胞分化培养的生物学特性
Biological characteristics of in vitro differentiation of bone marrow stromal cells into osteoblasts in rats
李雪峰 1刘亮 2王东2
作者信息
- 1. 北京市丰台区长辛店医院骨科,北京市,100043
- 2. 山西医科大学第二附属医院骨科,山西省太原市,030000
- 折叠
摘要
Abstract
BACKGROUND: Because of convenience and small injury to the body, bone marrow stromal cells (BMSCs) are the mostappropriate seed cells in bone tissue engineering. But the methods in vitro cultivation and the biological characteristic are notclear.OBJECTIVE: to establish a simple and effective BMSCs cultured in vitro and osteoblasts induction method, and to explore thebiological characteristics in vitro.METHODS: The BMSCs of Wistar rats were seperated and purified by using of adherent method. The passage cells of the thirdgeneration were cultured in the common medium (control group) and osteogenic induction medium (experimental group) for 10days, the growth cure of each group was drew. The alkaline phosphatase activities (APA) of two groups were detected at 4, 7, 10,13, 16, days’ culture and cover glass was treated with Von Kossa staining.RESULTS AND CONCLUSION: The better homogenicity of BMSCs were obtained by adherent method. The growth velocity ofthe cells cultured in the control group was obviously faster than that in the experimental group. The APA in control group wassignificantly lower than that in experimental group (P ＜ 0.05). The Von Kossa’ staining in the experimental group was positive andnegative in the control group. Adherent screening method is a simple and effective method to separate and purify BMSCs.BMSCs can be differentiated into osteoblasts in vitro.关键词
体外培养/骨髓间充质干细胞/生物学特性/诱导分化/干细胞培养分类
基础医学引用本文复制引用
李雪峰,刘亮,王东..大鼠骨髓间充质干细胞体外向成骨细胞分化培养的生物学特性[J].中国组织工程研究与临床康复,2011,15(23):4185-4188,4.
