中国组织工程研究与临床康复2011，Vol.15Issue(23)：4194-4198,5.DOI:10.3969/j.issn.1673-8225.2011.23.004
不同浓度重组人促红细胞生成素对神经干细胞体外培养增殖的影响
Effects of different concentrations of recombinant human erythropoietin on proliferation of neural stem cells cultured in vitro
薛政民 1胡萌 1张长海 1张宪成 1周晓鹏1
作者信息
- 1. 济宁市第二人民医院,山东省济宁市,272000
- 折叠
摘要
Abstract
BACKGROUND: Recombinant human erythropoietin (rhEPO) is a glycoprotein. Recent studies have demonstrated that rhEPO regulates many functional activities of neural cells. OBJECTIVE: To investigate the effects of different concentrations of rhEPO on proliferation of neural stem cells (NSCs) cultured in vitro. METHODS: Newborn Sprague-Dawley rat NSCs were harvested and cultured with serum-free culture medium containing different concentrations (5, 50, 500 U/mL) of rhEPO and 20 μg/L basic fibroblast growth factors (5, 50, and 500 U/mL rhEPO groups) and serum-free culture medium only containing 20 μg/L basic fibroblast growth factors (control group). After 7 days of culture, the cloning efficiency of NSCs was calculated. After 10 days of culture, neuron specific enolase (NSE)-and glial fibrillary acidic protein (GFAP)-immunoreactive cells were quantified. RESULTS AND CONCLUSION: In the rhEPO groups, cells proliferated rapidly, and the number of NSC microspheres was greater, in particular in the 50 U/mL rhEPO group, compared with the control group. NSCs grew faster in the 50 U/mL rhEPO group than in the control group. The number of NSE-and GFAP-immunoreactive cells was greater in the 50 U/mL rhEPO group than in the control group (P<0.01). These findings suggest that rhEPO promotes the in vitro culture and proliferation of NSCs, in particular 50 U/mL rhEPO.关键词
重组人促红细胞生成素/神经干细胞/碱性成纤维细胞生长因子/体外培养/增殖分类
医药卫生引用本文复制引用
薛政民,胡萌,张长海,张宪成,周晓鹏..不同浓度重组人促红细胞生成素对神经干细胞体外培养增殖的影响[J].中国组织工程研究与临床康复,2011,15(23):4194-4198,5.
