中国组织工程研究与临床康复2011，Vol.15Issue(23)：4215-4219,5.DOI:10.3969/j.issn.1673-8225.2011.23.009
海洋单细胞海藻体外对人脑胶质瘤干细胞生物学活性的影响
Marine phytoplankton effects on biological activity of human glioma stem cells in vitro
段小兵 1朱万万 2关方霞 3邓晓辉 3杨波 1张天祥 1田毅 2乔晓俊 1李远 1梁硕1
作者信息
- 1. 郑州大学第一附属医院神经外科,河南省郑州市,450052
- 2. 河南省高等学校临床医学重点学科开放实验室,河南省郑州市,450052
- 3. 郑州大学生物工程系,河南省郑州市,450001
- 折叠
摘要
Abstract
BACKGROUND: Marine phytoplankton (MPPT) has important guiding significance for application to strengthen the research. OBJECTIVE: To investigate the effect of MPPT on biologic activity of brain gliomastem cells (BGSCs) in vitro. METHODS: BGSCs cultured in the enzyme digestion way. The CD133 positive cells were sorted out by flow cytometry, and the 3rd passage of BGSCs were gathered through sub-culturing. CD133 expression was identified by flow cytometry before and after the effect of MPPT. Immunohistochemisty was used to detect Nestin and glial fibrillary acidic protein (GFAP) expression of adherent cells. The experimental group was added with different concentrations of MPPT, the negative control group was added with just PBS. 4, 6, 8, 10 g/L MPPT was added into the cell culture fluid. The cell cycle of BGSCs was inspected by flow cytometry, and inhibitory effects were detected using a microplate reader respectively at 24, 48, 72 hours later.RESULTS AND CONCLUSION: Compared to those in the control group, BGSCs in the experimental group could not form a compact, and began to be adherent and differentiated gradually observed by an inverted microscope, with the increasing of time and concentration. CD133 expression in BGSCs after the effect of MPPT was reduced obviously. The adherent cells expressed GFAP and Nestin. The cell number in S and G2/M phase was increased, and that in G0/G1 phase was decreased. Growth curve indicated that the proliferation of BGSCs was inhibited obviously, with the increasing of time and concentration (P ＜ 0.05-0.01). Results indicate MPTT can inhibit the proliferation of BGSCs, and promote them differentiate, which shows a dose and time dependent effect.关键词
海洋单细胞海藻/脑胶质瘤干细胞/CD133/巢蛋白/细胞周期分类
医药卫生引用本文复制引用
段小兵,朱万万,关方霞,邓晓辉,杨波,张天祥,田毅,乔晓俊,李远,梁硕..海洋单细胞海藻体外对人脑胶质瘤干细胞生物学活性的影响[J].中国组织工程研究与临床康复,2011,15(23):4215-4219,5.
