中国组织工程研究与临床康复2011，Vol.15Issue(23)：4247-4250,4.DOI:10.3969/j.issn.1673-8225.2011.23.016
脂肪源性干细胞成骨分化过程中成骨相关miRNA的表达模式
Osteogenesis-specific miRNA expression pattern analysis in osteogenic differentiation of adipose-derived stem cells
摘要
Abstract
BACKGROUND: miRNAs have emerged as important regulators in various physiological and pathological processes of cell differentiation, and can regulate the osteogenic differentiation of adipose-derived stem cells.OBJECTIVE: To screen the osteogenesis-specific miRNAs, and analyze the expression pattern of these miRNAs in osteogenic differentiation of adipose-derived stem cells.METHODS: Adipose-derived stem cells were isolated and cultured from human subcutaneous fat. The osteogenesis-specific miRNAs were screened by gene microarray technique. The relative expression of these miRNAs was analyzed on 7, 14, and 21 days by RT-PCR. The osteogenesis-specific proteins were detected on 7, 14, and 21 days by enzyme linked immunosorbent assay kit.RESULTS AND CONCLUSION: ①The 3rd passage adipose-derived stem cells were homogeneous. Osteogenesis,adipogenesis, and chondrogenesis differentiation of adipose-derived stem cells need specific condition under an inverted microscope. ②Nine osteogenesis-specific miRNAs were picked up by gene microarray technique, five were upregulated and four were downregulated. ③On day 7 in osteogenic differentiation, miR-106a expression was upregulated 1.58 folds (P ＜ 0.05). On day 14, nine miRNAs were upregulated. On day 21, five miRNAs were upregulated and four were downregulated. ④The concentration of osteogenesis-specific proteins such as osteocalcin, alkaline phosphatase, collagen Ⅰ and bone sialoprotein were increased on day 7, peaked on day 14 and slightly decreased on day 21.关键词
成骨分化/miRNA/脂肪来源干细胞/基因芯片/表达模式分类
医药卫生引用本文复制引用
张浩,康焱,马元琛,张紫机,黄保丁,廖威明..脂肪源性干细胞成骨分化过程中成骨相关miRNA的表达模式[J].中国组织工程研究与临床康复,2011,15(23):4247-4250,4.基金项目
国家自然科学基金(30872615),名称:双层一体Collagen/TCP梯度软骨修复材料生物响应特性的机理研究 （30872615）
丹麦战略研究基金(2101-07-0120),名称:Individualized Musculoskeletal Regeneration and Reconstruction Network. （2101-07-0120）
