安徽农业科学2011，Vol.39Issue(15)：8883-8884,8886,3.
微针注射甜玉米胚芽gus基因转化瞬时表达研究
Research of gus Gene Transformation Transient Expression on Microneedle Injection Sweet Corn(Z. mays L. ) Germ
刘立岩1
作者信息
- 1. 铁岭师范高等专科学校,辽宁铁岭,112000
- 折叠
摘要
Abstract
[ Objective ] The aim to study the gus gene transformation transient expression on microneedle injection sweet corn germ, to lay basis for deeply researching corn transformation. [ Method ] The experiment was done by using gus gene transient expression technology mediated by agrobacterium, and a embryo injection system for open gene transformation was built. [ Result ] The results showed that the seeds germ with the length of 3 mm was the most optimal transformation receptor. The optimal OD value of EHA105 agrobacterium of bacterial concentration was 0.558 -0.630. The optimal days of co-culture in gus gene transformation was 3 days. Under the optimal conditions, the microneedle injection of germ growth point gene conversion rate was 1％. [ Conclusion] The study can provide reference for improving the hereditary character and cultivating new varieties of genetically engineering of sweet corn.关键词
甜玉米/微针/注射/胚芽/基因转化/瞬时表达Key words
Sweet corn/ Microneedle/ Injection/ Germ/ Gene transformation/ Transient expression分类
农业科技引用本文复制引用
刘立岩..微针注射甜玉米胚芽gus基因转化瞬时表达研究[J].安徽农业科学,2011,39(15):8883-8884,8886,3.
