摘要
Abstract
[ Objective ] The aim was to isolate and identify aniline degrading bacterium with orthogonal test, and study its degradation characteristics. [ Method] Three aniline degrading bacterium were obtained from the soil sample which was collected from the circumambience of drainage ditch in a printing plant by isolation and screening, and the physical and chemical properties were determined, 16s rDNA sequence was analyzed. The effects of 5 factors included temperature, pH value, the anilines concentration, incubation time (hours) and the rotate speed on degradation rate of aniline degrading bacterium were examined. Regarding the degradation rate of aniline as the index, choosing temperature, pH value, the anilines concentration, incubation time (hours) and the rotate speed as factors, the degradating condition were optimized by orthogonal test. [ Result ] Three degrading bacterium were identified as Pseudomonas sp. , Acinetobacter gerneri and Delftia sp. ,named as AN-Y, AN-B and AN-H. The optimum temperature and pH value for growth and aniline degradation of three aniline degrading bacterium were 30 ℃ and 7.0, while they were capable of growing on the aniline substrate with the most consistency of aniline reached to 3 300,2 000 and 3 000 mg/L. The most degrading conditions of temperature was 30 ℃ , pH value was 8.0, the anilines concentration was 2 000 mg/L, rotate speed was 160 r/min and incubation time (hours) was 72 h. The variance analysis showed that the amount of aniline had significant effect on the degradation efficiency of degrading bacterium. [ Conclusion ] The study provides theoretical basis for the bacilliculture of aniline degrading bacterium.关键词
苯胺类化合物/降解菌/分离鉴定/降解特性Key words
Aniline categories compound/ Degrading bacterium/ Isolation and identification/ Degradation characteristics分类
农业科技
