中国免疫学杂志2011，Vol.27Issue(7)：583-587,5.DOI:10.3969/j.issn.1000-484X.2011.07.002
甲基乙二醛对Jurkat细胞氧化应激及分泌细胞因子的影响
Effects of methylglyoxal on oxidative stress and cytokine profiles in cultured Jurkat cells
摘要
Abstract
Objective:To investigate the effects of mthylglyoxal(MGO) on oxidative stress and cytokine profiles in cultured Jurkat cells for understanding more about the mechanism of diabetes accelerated athevosclerosis.Method:Jurkat cell which pre-stimulated by PHA were incubated with MGO for 24h.The cells survial rate was detected by MTT.ROS was quantitated by flow cytometry.Total and phosphory-lated p38 and JNK were assessed using Western blot.After incubation with MGO,the expression of TNFF-α and IFN-γ were tested by ELISA.Results:No changes in cells viability was observed when Jurkat cells were treated with 0-60 μmol/1<MGO.Incubation of Jurkat cells with MGO significantly induced ROS production.Co-incubation with NAC or AG decreased MGO-induced ROS production.Incubation of Jurkat cells with MGO results in significant increase in p38 and JNK phosphorylation.In addition MGO significantly increased the production of TNF-α and IFN-γ while p38,JNK inhibitor and NAC reversed the secretion of pro-inflammatory cytokines.Conclusion:These data demonstrate that MGO in-duced the release of TNF-α and IFN-γ in Jurkat cells viaoxidative stress,p38,JNK signaling pathway.关键词
甲基乙二醛/Jurkat细胞/氧化应激/细胞因子/动脉硬化Key words
Methylglyoxal/Jurkat cells/Oxidative stress/Cytokines分类
医药卫生引用本文复制引用
游捷,余洪根,彭雪峰,刘晓红,刘礼斌..甲基乙二醛对Jurkat细胞氧化应激及分泌细胞因子的影响[J].中国免疫学杂志,2011,27(7):583-587,5.基金项目
本文为福建省自然科学基金计划资助项目(C0710017) （C0710017）
