中国免疫学杂志2011，Vol.27Issue(7)：602-606,615,6.DOI:10.3969/j.issn.1000-484X.2011.07.006
根皮素对人γδT细胞杀伤胃癌SGC-7901细胞的影响及机制探讨
Explore the effect and mechanism of phloretin on γδT cells kill gastric cancer SGC-7901 cells
摘要
Abstract
Objective:To explore the effect and mechanism of phloretin on γδT cells kill human gastric cancer SGC-7901 cells.Meth-ods:γδT cells were amplified from human peripheral blood cells human peripheral blood cells through IPP method.After cultured with different concentrations of phloretin for 48 hours,MTT method was used to test the growth curve of γδT cells and SGC-7901 cells;FCM was used to test the expression of PFP and G raB of γδT cells;LDH release method was used to test the cell-killing activity of γδT cells to SGC-7901 cells;Western blot to test the Wnt3a expres-sion of γδT cells.Results:After cultured with IPP for 10 days,γδT cells increased from 3.12％ to 79.6％.Compared to the control group,when the concentration of phloretin increased from 2.35μg/ml to 18.75μg/ml it could proliferate the γδT cell growth significantly(p＜0.05) and phloretin at concentration 75μg/ml could inhibit the gastric cancer SGC-7901 cell growth significantly(p＜0.05).When the concentration in-creased from 2.35μg/ml to 75μg/ml the killing activity of γδT cells to the SGC-7901 cells enhanced significantly(p＜0.05);the expression of PFP,GraB and Wnt-3a increased significantly compared to the control group(p＜0.05).Conclusion:Phloretin can enhance the killing ef-fect γδT cells to SGC-7901 cells,the mechanism may be related to the phloretin could proliferate the γδT cell growth,incerase the expression of PFP,GraB and actived the Wnt signaling pathway.关键词
根皮素/γδT细胞/胃癌/杀伤活性Key words
Phloretin/γδT cells/Gastric cancer/Killing activity分类
医药卫生引用本文复制引用
刘刚,陈复兴,刘军权,吕小婷,费素娟..根皮素对人γδT细胞杀伤胃癌SGC-7901细胞的影响及机制探讨[J].中国免疫学杂志,2011,27(7):602-606,615,6.基金项目
本文受南京军区医学科技创新经费资助项目(09MA037)资助 （09MA037）
