中国免疫学杂志2011，Vol.27Issue(7)：625-629,5.DOI:10.3969/j.issn.1000-484X.2011.07.011
脐血CD4+CD45+调节性T细胞体外扩增及其抑制复发性流产患者免疫细胞功能研究
Amplification of cord blood CD4+CD45+ regulatory T cells in vitro and its inhibition to the immunity of recurrent spontaneous abortion
摘要
Abstract
Objective:To establish a practial and convenient approach of CD4+CD5+ regulatory T cells expansion in vitro and in-vestigate the effect on the immunity of recurrent spontaneous abrtion.Methods:CD4+CD5+ regulatory T cells were isolated from cord blood by MACS and then expand in vitro.Frequency and phenotype of Treg cells was determined by FACS.The inhibitory effect of expanded on effector T cell and NK cells was tested by MTT and 51Crreleasing method,respectively.Results:The amplification multiples of Treg cells were 6.5±0.6 folds,18.2±2.5 folds,and 52.7±4.8 folds,and the purity of Treg cells were (93.2±3.4)％,(88.6±2.9)％and (81.9±3.3)％,re-spectively.It was shown by the inhibitory test that the expanded Treg cells could inhibit the proliferation of CD4+CD5- T cells and the specificlysis of NK cells in peripheral blood of RSA.The expanded Treg cells could down-regulate the level of IFN-γ and up-regulate the level of IL-10 in peripheral blood of RSA.Conclusion:The expanded Treg cells could significantly inhibit the immunity of recurrent spontaneous abortion.关键词
CD4+CD25+Treg/体外扩增/复发性流产Key words
CD4+CD5+ Treg/Amplification in vitro/Recurrent abortion分类
医药卫生引用本文复制引用
秦卫兵,崔蓉,杨宁,王一峰,苗竹林,韦相才,欧汝强..脐血CD4+CD45+调节性T细胞体外扩增及其抑制复发性流产患者免疫细胞功能研究[J].中国免疫学杂志,2011,27(7):625-629,5.基金项目
本文为广东省科技计划项目(No.2008B03031193)和国家自然科学基金项目(No.30901610)资助课题 （No.2008B03031193）
