中国免疫学杂志2011，Vol.27Issue(7)：639-641,647,4.DOI:10.3969/j.issn.1000-484X.2011.07.014
一株A和B亚群禽白血病病毒特异性单克隆抗体的制备及其特性
Development and characterization of monoclonal antibody to subgroup A and B avian leukosis virus
摘要
Abstract
Objective:Development and characterization of monoclonal antibody to subgroup A and B avian leukosis virus.Methods:The env-gp85 gene of ALV-A-SDAU09C1 was subcloned into the prokaryotic expressing vector pET32a and recombinant vector pET32a-SDAU09C1-gp85 was transformed into Escherichia coli strain BL21 for expression under the induction of IPTG.After IPTG induction,there was a new protein band about 53 kD on SDS-PAGE.The expressed proteins were vaccinated into Balb/c mice.Results:One Mab(A6D1),which reacted with all exogenous subgroups of AL-AB but not with endogenous subgroup J,was obtained.Using immunofluorescence assay(IFA),A6D1 reacted with 3 ALV-A and 1 ALV-B ,but not with 4 ALV-J.Western B lot showed that molecular weight of ALV AB enve-lope glycoprotein or unglycosylated envelope protein recognized by Mabs was about 53kD.Conclusion:A6D1 can be used for diagnosis and epidemology of ALV-AB,which compensated for only the subgroup JALV could be used in clinical.关键词
禽白血病病毒/AB/亚群/囊膜蛋白/单克隆抗体Key words
Avian leukosis virus/Subgroup AB/Envelope protein/Monoclonal antibody分类
农业科技引用本文复制引用
邱玉玉,李晓霞,武专昌,崔治中,孙淑红,张显忠..一株A和B亚群禽白血病病毒特异性单克隆抗体的制备及其特性[J].中国免疫学杂志,2011,27(7):639-641,647,4.基金项目
本文受国家公益性行业重大项目(#200803019)、山东省科技攻关项目(#2010GNC10924)和泰山医学院学校科研计划项目(#2010ZR070)等资助 （#200803019）
