中国组织工程研究与临床康复2011，Vol.15Issue(23)：4272-4276,5.DOI:10.3969/j.issn.1673-8225.2011.23.021
黄芪多糖对2型糖尿病患者外周血内皮祖细胞PI3K/Akt/eNOS信号通路的影响
Effect of astragalus polysaccharides on peripheral endothelial progenitor cells via PI3K/Akt/eNOS signal pathway in patients with type 2 diabetes
摘要
Abstract
BACKGROUND: Previous studies have demonstrated that Astragalus can promote the proliferation of endothelial progenitor cells (EPCs) via P38MAPK pathway. Whether PI3K/Akt/eNOS signal pathway can replace P38MAPK pathway needs further studies. OBJECTIVE: To observe the effect of astragalus polysaccharides (APS) on the expression of protein kinase B (PKB) and endothelial nitric oxide synthase (eNOS) of EPCs from peripheral blood in patients with type 2 diabetes.METHODS: Mononuclear cells from diabetic patients were isolated by Ficoll density gradient centrifugation, and identified as EPCs after 7 days. Dose-depended and time-depended effects of APS with six different concentrations (0, 50, 200, 800, 3 200, 6 400 mg/L) and different treatment time (6, 12, 24, 48 hours) on EPCs proliferation and differentiation were measured. EPCs were treated with APS alone or APS combined with LY294002 to observe the expressions phosphorylated Akt and eNOS using Western Blot. EPCs from healthy persons were used as controls.RESULTS AND CONCLUSION: The proliferative ability of EPCs in the diabetic group was significantly lower than that in the control group (P ＜ 0.05). 200-800 mg/L APS within 6-24 hours could promote the proliferative ability of EPCs in a time- and dose-depended manner (P ＜ 0.01). With dose increasing, the expressions of phosphorylated Akt and eNOS were also increased (P ＜ 0.05). LY294002 could inhibit the phosphorylation of Akt and eNOS (P ＜ 0.05). The findings indicate that APS can promotethe proliferation and differentiation of EPCs into endothelial cells via activated PI3K/Akt/eNOS pathway.关键词
黄芪多糖/内皮祖细胞/内皮型一氧化氮合酶/磷脂酰肌醇-3激酶/蛋白激酶B分类
医药卫生引用本文复制引用
徐寒松,吴青,谢晓云,孔德明..黄芪多糖对2型糖尿病患者外周血内皮祖细胞PI3K/Akt/eNOS信号通路的影响[J].中国组织工程研究与临床康复,2011,15(23):4272-4276,5.基金项目
国家自然科学基金资助项目(30960491) （30960491）
贵州省优秀科技教育人才省长基金资助项目[黔省专合字(2009)84号]. （2009）
