中国免疫学杂志2011，Vol.27Issue(7)：648-653,6.DOI:10.3969/j.issn.1000-484X.2011.07.016
抗人CD25嵌合抗体基因的构建及其瞬时表达研究
Study on construction and transient expression of human-mouse chimeric antibody gene against human CD25
胡迪超 1张爱华 1潘勇兵 1詹珊珊 1杨晓明1
作者信息
摘要
Abstract
Objective:To construct chimeric antibody gene against human an CD25 angigen,and prelin inarily identify the expressed prod-ucts produced from transiently transfected mammalian cells in order to facilitate the further study of stable expression.Methods:The RLM-RACE was employed to clone variable region genes and leader sequences,and the Overlap PVR method was used to construct the chimeric anti-body gene.After transiently transfected in three mammalian cells with liposome method, the expressed products were determined by ELISA,FCM,W B,Dotblot and immunofluorescence assay.Results:The variable region genes and leader sequences were successfully amplified,and the eukayotic expression plasmids were constructed.The results from transient transfection indicate the expressed products retain the antigen binding capacity of parental antibody WuTac.Conclusion:The successfully constructed chimeric antibody gene against human CD25 lays sound foun-dation for further study.关键词
CD25/RLM-RACE/基因拼接/嵌合抗体/瞬时表达Key words
CD25/RLM-RACE/Gene splicing/Chimeric antibody/Transient expression分类
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胡迪超,张爱华,潘勇兵,詹珊珊,杨晓明..抗人CD25嵌合抗体基因的构建及其瞬时表达研究[J].中国免疫学杂志,2011,27(7):648-653,6.
