中国免疫学杂志2011，Vol.27Issue(7)：654-656,3.DOI:10.3969/j.issn.1000-484X.2011.07.017
IL-18与HSV-2gD模拟抗原表位DNA疫苗的免疫效果观察
The immune responses of the DNA vaccine of IL-18 and HSV-2gD
摘要
Abstract
Objective:To study the immune responses of pcDNA3.1-IL-18-HSV P6 and pcDNA 3.1-IL-18-HSV NP6 genetic vaccine.NP6 is the similar sequence of natural HSV-2 gD from gene bank.Methods:The recombinant plasmid pcDNA3.1-IL-18、pcDNA3.1-IL-18-HSV P6 and pcDNA3.1-IL-18-HSV NP6 were used to inoculate BALB/c mice by intramuscular injection for three times,once a week.One week after the last vaccination,the levels of specific IgG antibody,IFN-γ and IL-18 were detected by ELISA;One month after the last vaccina-tion,spleen cells of vaccinated mice were separated and proliferation of spleen lymphocytes was detected by MTT.Results:After inoculated by pcDNA3.1-IL-18-HSV P6 vaccine,the mice could produce higher level of splenocytes proliferation and secrect IFN-γ and IL-18 and specific an-tibody.Conclusion:the recombinant pcDNA3.1-IL-18-P6 can effectively induce humoral and cellular immune responses,which offered the basic requirements for constructing new type of DNA vaccine.关键词
DNA疫苗/IL-18/单纯疱疹病毒Ⅱ型/模拟抗原表位Key words
DNA vaccine/Interleukin-18/HSV-Ⅱ/M inotope分类
医药卫生引用本文复制引用
焦凤萍,刘莉,于爱莲,王玉,于广福..IL-18与HSV-2gD模拟抗原表位DNA疫苗的免疫效果观察[J].中国免疫学杂志,2011,27(7):654-656,3.基金项目
本文为山东省卫生厅资助课题(2007HZ037)和山东省自然科学基金(ZR2009CQ003)资助项目 （2007HZ037）
