中国组织工程研究与临床康复2011，Vol.15Issue(23)：4316-4320,5.DOI:10.3969/j.issn.1673-8225.2011.23.031
静脉移植CXCR-4基因转染骨髓间充质干细胞在脊髓损伤鼠体内的迁移与分化
Immigration and differentiation of bone marrow mesenchymal stem cells transfected by CXCR-4 gene after intravenous transplantation in mice with spinal cord injury
摘要
Abstract
BACKGROUND: Previous studies demonstrated that stromal cell derived factor-1/CXCR-4 axis system plays a role in mediating bone marrow mesenchymal stem cells’ (BMSCs) orient immigration.OBJECTIVE: To investigate the feasibility of BMSCs transfected by CXCR-4 gene after intravenous transplantation for treatment of spinal cord injury (SCI).METHODS: C57BL/6 mice T10 SCI model was constructed by spinal cord transection method, and at 7 days after modeling, which were randomly divided into 3 groups: experimental group, control group and blank group. Mice were intravenous injected by the suspensions of allogeneic BMSCs transfected by CXCR-4 gene in experimental group. Mice were respectively intravenous injected by the suspensions of non-transfected allogeneic BMSCs and non-cell culture medium in control and blank groups. At 7, 14, 21, 28 days after transplantation, the survival and differentiation of green fluorescence protein positive cells to all cells in different site after transplantation were detected by laser scanning confocal microscope and Immunohistochemical double staining method. BBB score was used to evaluate the recovery condition of neuromotor function of mice.RESULTS AND CONCLUSION: After transplantation, green fluorescence protein positive cells significantly gathered in the injured part of spinal cord in experimental group at each time point, and the number gradually increased, and the transport factor was obviously higher than in control and blank groups. The proportion of neural cells from BMSCs in transplanted cells in experimental group was also higher than that in control and blank groups. The neuromotor function of mice recovered obviously. BMSCs transfected by CXCR-4 gene after intravenous transplantation showed stronger orient immigration ability to SCI focus, which can survive and differentiate in the SCI focus, and play a role in repairing SCI.关键词
定向迁移/间充质干细胞/CXCR-4基因/脊髓损伤/转染/移植分类
医药卫生引用本文复制引用
宋晨阳,徐皓,陈建梅,潘廷明,姚晓东..静脉移植CXCR-4基因转染骨髓间充质干细胞在脊髓损伤鼠体内的迁移与分化[J].中国组织工程研究与临床康复,2011,15(23):4316-4320,5.基金项目
南京军区"十一?五"医药卫生科研重点基金资助项目(07Z037),课题名称:调控SDF-1/CXCR-4促进神经样BMSCs定向迁移修复脊髓损伤的实验研究. （07Z037）
