中国组织工程研究与临床康复2011，Vol.15Issue(23)：4325-4329,5.DOI:10.3969/j.issn.1673-8225.2011.23.033
一定浓度下三氧化二砷对T细胞淋巴瘤Hut-78细胞株增殖的影响
Effect of arsenic trioxide on proliferation of T cell lymphoma cell line Hut-78
摘要
Abstract
BACKGROUND: In recent years, although some effective therapies of arsenic trioxide for T cell lymphoma have been reported, this mechanism is yet unknown.OBJECTIVE: To investigate the effect of arsenic trioxide on inhibiting proliferation, inducing apoptosis and cell cycle of T cell lymphoma cell line Hut-78 cells.METHODS: The inhibitory effect of arsenic trioxide (2, 5, 10 μmol/L for 24, 48, 72 hours) on proliferation and its inducing apoptosis effect, influence on cell cycle of Hut-78 cell line were detected by MTT, flow cytometry with PI staining and terminal deoxynucleotidy transferase-mediated dUTP nick end-labeling (TUNEL) assay. The transcription level changes of bcl-2 gene and vascular endothelial growth factor (VEGF) gene was measured by RT-PCR. RESULTS AND CONCLUSION: In the concentration range of 2-10 μm/L of arsenic trioxide, with the increasing of concentration and extending of action time, the growth inhibition of Hut-78 cells was gradually increased. The apoptosis of Hut-78 mainly occurred at G2-M phase. The effect of arsenic trioxide to induce Hut-78 apoptosis could be related to down-regulation of bcl-2 gene. Arsenic trioxide has an influence on inhibiting proliferation and inducing apoptosis of Hut-78 in a time- and dose-depended manner.关键词
三氧化二砷/T细胞淋巴瘤/细胞增殖/细胞凋亡/bcl-2基因分类
医药卫生引用本文复制引用
张学美,王婷,李晓进,赵亮,李惠民..一定浓度下三氧化二砷对T细胞淋巴瘤Hut-78细胞株增殖的影响[J].中国组织工程研究与临床康复,2011,15(23):4325-4329,5.基金项目
昆明医学院研究生创新基金项目(KM2008L55)"淋巴瘤小鼠模型的建立及三氧化二砷抑瘤机制的探讨". （KM2008L55）
