中国组织工程研究与临床康复2011，Vol.15Issue(23)：4344-4350,7.DOI:10.3969/j.issn.1673-8225.2011.23.038
诱导多功能干细胞的研究进展
Progress regarding induction of pluripotent stem cells
摘要
Abstract
BACKGROUND: There are large research and clinical application limitations on embryonic stem cells (ESCs) considering its potential oncogenicity and ethical disputes. In 2006, Yamanaka found that four factors (Oct3/4, Sox2, Klf4, c-Myc) could induce mouse embryonic fibroblasts into an embryonic stem cell-like state, which is called induced pluripotent stem cells (iPSCs). This shows the appearance of a new kind of embryonic stem cell-like cells.OBJECTIVE: To understand progress and application of iPSCs in the field of stem cells.METHODS: The first author searched PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) and Wanfang database (http://g.wanfangdata.com.cn/) for literatures concerning iPSCs generation, characteristics, and research progress from 2006 to 2010. Key words were “induced pluripotent stem cells, defined factors, reprogramming, vectors, disease”. Duplicate articles were excluded. Finally, 69 articles were selected.RESULTS AND CONCLUSION: Great progress has been made on iPSCs, includes the kind of factors, vectors, reprogramming efficiency and application research. However, there are some obstacles on somatic cells reprogramming such as potential damage of exogenous factors and vectors to cells low reprogramming efficiency. Once these problems solved, iPSCs can be widely used for disease modeling, drug test, cell transplantation and production of patient-specific iPSCs.关键词
诱导多功能干细胞/重编程/因子/载体/效率分类
医药卫生引用本文复制引用
王格,陈哲,武栋成..诱导多功能干细胞的研究进展[J].中国组织工程研究与临床康复,2011,15(23):4344-4350,7.基金项目
国家自然科学基金项目(面上项目,30670813). （面上项目,30670813）
