中国组织工程研究与临床康复2011，Vol.15Issue(23)：4351-4354,4.DOI:10.3969/j.issn.1673-8225.2011.23.039
两种血细胞分离机干/祖细胞采集和移植效果的比较
Comparison of two blood cell separators in collection of peripheral blood stem cell/progenitor cells and effectiveness of transplantation
叶书来 1应美爱 2陈家萍 2耿良权3
作者信息
- 1. 安徽省立医院南区检验科,安徽省合肥市,230001
- 2. 安徽省立医院输血科,安徽省合肥市,230001
- 3. 安徽省立医院血液科,安徽省合肥市,230001
- 折叠
摘要
Abstract
BACKGROUND: Peripheral blood stem cells/progenitor cells were collected in vitro using blood cell separators, which directly affects the features and quantity of collected cells, additionally, affects hematopoietic reconstitution of recipients.OBJECTIVE: To evaluate the efficiencies of Fenwal CS-3000 Plus and Amicus blood cell separators in collecting peripheral blood stem cells/progenitor cells and effectiveness of transplantation.METHODS: Totally 51 cases received allogenic peripheral blood stem cells transplantation were selected, including 27 cases,mean aged (34.2±10.6) years, who performed stem cell apheresis procedures using Fenwal CS-3000 Plus blood cell separator and 24 donors, mean aged (35.4±12.1) years, using Amicus blood cell separator. The number of nucleated cells and CD34+ cells,collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution of two devices were compared.RESULTS AND CONCLUSION: There were no significant differences in the total number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution between the two devices. However, the amount of platelet in the stem/progenitor cell products of the Fenwal CS-3000Plus group was obviously greater than that of the Amicus group (P ＜ 0.01). There is no difference in the collection efficiencies of the two blood cell separators in the peripheral blood stem cells/progenitor cell and the effectiveness of transplantation. The Amicus cell separator is better for the donors with thrombocytopenia.关键词
血细胞分离机/外周血造血干/祖细胞/CD34+细胞/分离效果/移植分类
基础医学引用本文复制引用
叶书来,应美爱,陈家萍,耿良权..两种血细胞分离机干/祖细胞采集和移植效果的比较[J].中国组织工程研究与临床康复,2011,15(23):4351-4354,4.
