作物学报2011，Vol.37Issue(5)：745-754,10.DOI:10.3724/SP.J.1006.2011.00745
分子标记辅助选择改良武运粳8号的条纹叶枯病抗性
Improving the Resistance of Wuyunjing 8 to Rice Stripe Virus via Molecular Marker-assisted Selection
摘要
Abstract
In this study, a molecular marker-assisted selection (MAS) strategy was used to improve Wuyunjing 8's resistance to rice stripe virus. The Wuyunjing 8 with high yield and erect head selected from 25 late-maturing varieties was used as accepter,and the variety Kuifeng with resistance to rice stripe virus as the donor. Four STS (sequence-tagged site) primers STS11-31,STS 11-7, STS 11-19, and STS 11-43 anchoring the resistance genes were used for assisted selection in the backcross progenies. To 2008, seventy lines in BC3F5 and one hundred and fifteen lines of BC4F4 with stable agronomic traits were obtained with the result of molecular analysis. By appraising agronomic traits, yield, quality and resistance in backcross progenies, teen lines from BC4F5 and two lines from BC3F6, which all carried disease-resistant genes and were similar to Wuyunjing 8 on the integrated traits, were selected. These lines retain the characteristics of high yield and good quality of Wuyunjing 8 and have been prominently improved in resistance to rice stripe virus.关键词
粳稻/条纹叶枯病/抗性/改良/分子标记辅助选择Key words
Japonica/ Rice stripe virus/ Resistance/ Improvement/ Molecular marker-assisted selection引用本文复制引用
张宏根,梁国华,顾铭洪,许作鹏,李鹏,李波,刘超,朱邦辉,徐洁芬,钮中一,汤述翥..分子标记辅助选择改良武运粳8号的条纹叶枯病抗性[J].作物学报,2011,37(5):745-754,10.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2006CB101701,2006CB101703)和国家转基因生物新品种培育重大专项(2008ZX08001002)资助. （973计划）
