华东师范大学学报（自然科学版）Issue(2)：134-141,8.DOI:10.3969/j.issn.1000-5641.2011.02.017
视黄酸对热带爪蟾胚胎致畸效应的表型特征
Teratogenic phenotypes induced in Xenopus tropicalis embryos by all-trans retinoic acid
摘要
Abstract
In this study, Xenopus tropicalis embryos were exposed to a known teratoen, alltrans retinoic acid (RA), for 24, 36 and 48 h. Exposure to 2, 10 and 50 μg · L- 1 RA showed no effects on the survival rate of embryos, but it significantly inhibited the growth and development of embryos. RA also led to malformations in all embryos, which suggests that RA is a strong teratogen, and X. tropicalis is very sensitive to RA. The most characteristic malformations were small brains, less pigmentation in eyes, pericardial edema and bent tails. After 24, 36 and 48 h of exposure, the brain teratogenic index and eye teratogenic index were significantly increased in the treatment groups, and these effects showed a good concentration-effect but not time-effect relation. The tail teratogenic index was higher in embryos after 48 h of exposure than that after 24 h of exposure. The comparisons of teratogenic phenotypes induced by RA and tributyltin indicate that RA is a good reference for revealing the mechanisms of tributyltin-mediated teratogenicity. Our results also suggest that FETAX might be modified as an assay to study the mechanisms of contaminants in future.关键词
视黄酸/爪蟾/胚胎/致畸Key words
retinoic acid/Xenopus tropicalis / embryos/ teratogenesis分类
农业科技引用本文复制引用
曹庆珍,钱丽娟,张小利,袁静,刘俊奇,施华宏..视黄酸对热带爪蟾胚胎致畸效应的表型特征[J].华东师范大学学报（自然科学版）,2011,(2):134-141,8.基金项目
国家自然科学基金(20877023) （20877023）
上海市自然科学基金(08ZR1406900) （08ZR1406900）
长江水环境教育部重点实验室开放基金(YRWEF06003) （YRWEF06003）
