中国人兽共患病学报2011，Vol.27Issue(6)：470-474,5.
黑龙江立克次体感染血管内皮细胞的初步研究
Primary study on Rickettsia heilongjiangensis infection of vascular endothelial cells
摘要
Abstract
The Rickettsia heilongjiangensis organisms purified by Renografin renografin gradient centrifugation were applied to infected human umbilical vein endothelial cells (HUVEC) grown in monolayer on cover slips. Rickettsiae were examined by both indirect immune fluorescence assay (IFA) and scanning electron micrograph (SEM) after rickettsial infection.During the first 24 hours postinfection (ip), two infection peaks were found at 6 and 24 hours pi, respectively. Multiplication of rickettsiae and pathological changes of host cells were observed at 5 days pi, while the amount of intracellular rickettsiae was markedly increased within 5 to 9 days pi. A few of rickettsiae were observed in the nucleus of host cells that appeared severe pathological changes within 8 to 9 days pi. Due to the overwhelming rickettsial infection, most of the infected host cells shed 12 days pi. Our results demonstrated that R. heilongjiangensis has a capability to infect vascular endothelial cells to cause vascular injury of humans.关键词
黑龙江立克次体/血管内皮细胞/细胞病变Key words
Rickettsia heilongjiangensis/ vascular endothelial cells/ pathological change分类
医药卫生引用本文复制引用
孟艳芬,段长松,王锡乐,熊小路,温博海..黑龙江立克次体感染血管内皮细胞的初步研究[J].中国人兽共患病学报,2011,27(6):470-474,5.基金项目
国家基础研究计划(973计划课题2010CB530200/2010CB530205)和国家科技重大专项(重大传染病预防与控制课题2008ZX10004-002)联合资助 （973计划课题2010CB530200/2010CB530205）
