中国人兽共患病学报2011，Vol.27Issue(6)：475-478,483,5.
安氏隐孢子虫在HCT-8和AGS细胞中增殖效果的研究
In vitro proliferation of Cryptosporidium andersoni in HCT-8 and AGS cell lines
摘要
Abstract
In order to study the proliferation of Cryptosporidium andersoni in human ileocecal adenocarcinoma (HCT-8)cells and human stomach adenocarcinoma (AGS) cells, HCT-8 and AGS cells were harvested at mid-exponential phase and planted in 6 well plates. When the monolayers reached 60 -70％ confluency, the excysted oocysts (2.5 × l05/well) were inoculated into cells and parasite growth was terminated at 24 h and 48 h. Then the proliferation of parasites was determined by Giemsa staining and Real-time polymerase chain reaction (PCR) assay. At 24 h and 48 h, the number of parasites in HCT-8 cells was higher than that of AGS cells (P＜0.05) by Giemsa staining. The copies of COWP gene in HCT-8 cells were also higher than those of AGS cells (P＜0. 05) by Real-time PCR assay. It's found that the HCT-8 cell line was a more suitable host model to support Cryptosporidium andersoni proliferation than that of AGS cell line.关键词
安氏隐孢子虫/HCT-8细胞/AGS细胞/Giemsa染色/Real-time PCRKey words
Cryptosporidium andersoni/ HCT-8 cell/ AGS cell/ Giemsa staining/ Real-time PCR分类
医药卫生引用本文复制引用
逯兆喜,吴亮,姜旭淦,沈玉娟,傅行礼,涂国华,李礼,陈盛霞,曹建平..安氏隐孢子虫在HCT-8和AGS细胞中增殖效果的研究[J].中国人兽共患病学报,2011,27(6):475-478,483,5.基金项目
卫生部寄生虫病原与媒介生物学重点实验室开放课题(No.WS-BKFKT200701),国家卫生行业科研专项(No.200802012),国家传染病重大科技专项(No.2008ZX10004-002,No.2009ZX10004-201)和江苏大学科技创新团队项目(No.2008-018-02)联合资助 （No.WS-BKFKT200701）
