中国人兽共患病学报2011，Vol.27Issue(6)：479-483,5.
人伤寒沙门菌Ty21a菌蜕的制备
Preparation and identification of Salmonella enterica Ty21a bacterial ghosts
摘要
Abstract
In order to prepare Ty21a bacterial ghosts accomplished by the temperature-controlled expression of bacteriophage PhiX174 lysis gene E, two recombinant lysis plasmids were constructed by cloning the lysis gene E into temperature-induced plasmid system, and their lysis efficiency to E. coli DH5α was compared by counting the colony forming unit (CFU).Plasmid pMBE was transformed into bacteria Ty21a using improved transformation condition, then Ty21a bacterial ghosts (Ty21a BGs) were produced by shifting the temperature from 28℃ to 42℃. Ty21a BGs were harvested by centrifugation, and the lysis rate was calculated. The characterization of BG was observed by scanning electron micrograph (SEM) and transmission electron micrograph (TEM). The lysis rate of E. coli ghosts which were induced by plasmid pMBE was about 99. 99％.Bacteria Ty21a was efficiently transformed using optimal electroporation parameters (voltage 500V, electric resistance 200Ω,and capacitance 25μF), forming recombinant Ty21a (pMBE). Ty21a BGs were produced by controlled expression of PhiX174 lysis gene E, and the lysis rate was 96.77％. Ghosts show loss of cytoplasmic material and structural integrity. The Ty21a bacterial ghost was successfully produced by the controlled expression of PhiX174 lysis gene E, which provides an efficient delivery system for vaccine development.关键词
Ty21a菌/细菌菌蜕/电击转化/噬菌体PhiX174/E基因Key words
Ty21a/bacterial ghost/electroporation condition/PhiX174 lysis gene E分类
医药卫生引用本文复制引用
温晶,寇志华,于虹,吕兴茹,王欣,郭彦,俞建萍,赵光宇,周育森..人伤寒沙门菌Ty21a菌蜕的制备[J].中国人兽共患病学报,2011,27(6):479-483,5.基金项目
国家自然科学基金(30872392)和863课题(2007AA02Z414)联合资助 （30872392）
