神经损伤与功能重建2011，Vol.6Issue(3)：157-160,4.DOI:10.3870/sjsscj.2011.03.001
无饲养层条件原代培养小鼠胚胎干细胞的实验研究
Culture of Mural Embryonic Stem Cells Under Feeder-free Condition
刘登华 1张苏明 1闫秋月 1蔡俐琼2
作者信息
- 1. 华中科技大学同济医学院附属同济医院神经科,武汉,430030
- 2. 华中科技大学同济医学院附属协和医院妇产科,武汉,430022
- 折叠
摘要
Abstract
Objective: To study the isolation and primary culture of mural embryonic stem cells (ESC)under feeder-free condition. Methods:With the microdrop culture technique, blastocysts were cultured under feeder-free condition and on HEF feeder layer respectively. Development of blastocysts and their behavior of praxiology were observed and the ESC isolation efficiency under feeder-free condition and on HEF feeder layer was studied simultaneously. Results:Blastocysts could normally develop under both feeder-free condition and on HEF feeder layer. Primary ESC could be isolated by the two culture systems. When compared with the group of HEF feeder layer, attachment rate of blastocysts after 48 h culture and ESC isolation efficiency were significantly lower under feeder-free condition(P＜0.05). Conclusion: ESC can be primarily obtained under feeder-free condition. How to improve the ESC isolation efficiency needs further studies.关键词
小鼠/胚胎干细胞/分离培养/无饲养层Key words
mouse/embryonic stem cell/isolation and culture/ feeder-free分类
医药卫生引用本文复制引用
刘登华,张苏明,闫秋月,蔡俐琼..无饲养层条件原代培养小鼠胚胎干细胞的实验研究[J].神经损伤与功能重建,2011,6(3):157-160,4.
