热带作物学报2011，Vol.32Issue(2)：240-244,5.
橡胶树14-3-3蛋白基因的酵母双杂交诱饵载体的构建及自激活和毒性检测
Construction of Bait Vector Containing 14-3-3 Protein Gene from Hevea brasiliensis in Yeast Two-hybrid System and Identification of Its Toxicity and Autonomous Activation Effects
摘要
Abstract
Three genes encoding 14-3-3 protein, designated HbGF14a、 HbGF14b、 HbGF14c were amplified from Hevea brasiliensis by the PCR and then fused with pGBKT7 vector.After confirmed by using sequence analysis,the plasmids were transformed into the cell of yeast strain Y2H Gold, and tested in terms of toxicity and transcriptional activation by nutrition disfigurement assay.As a result, three genes were successfully amplified and cloned into the pGBKT7 vector.The yeast strain Y2H Gold cells harboring bait plasmids grew well on SD/-Trp plate, without toxicity and autonomous activation effect, which lays the foundation for using yeast two-hybrid system to screen the protein interacting with 14-3-3 protein.关键词
橡胶树/14-3-3蛋白/酵母双杂交/诱饵载体Key words
14-3-3 protein/ Hevea brasiliensis/ Yeast two-hybrid/ Bait vector分类
农业科技引用本文复制引用
杨子平,李辉亮,郭冬,彭世清..橡胶树14-3-3蛋白基因的酵母双杂交诱饵载体的构建及自激活和毒性检测[J].热带作物学报,2011,32(2):240-244,5.基金项目
国家自然科学基金(No.30960307) （No.30960307）
中央级公益性科研院所基本科研业务费专项资金项目(No.CATAS-ITBB110205). （No.CATAS-ITBB110205）
