热带作物学报2011，Vol.32Issue(2)：269-272,4.
东方百合高效离体再生体系的建立
Establishment of an Efficient Micropropagation System for Oriental Hybrid Lily
摘要
Abstract
A two-step explant method combined with embryogenic callus formation which could greatly increase regeneration efficiency of Oriental Hybrid lily was developed. Bulb scale segments were used as original explants and shoot sections excised from shoot clusters were used as the secondary expLants.ln addition,the effect of list range of 2,4-D(2,4-Dichlorophenoxyacetic acid) concentration and the effect of different kinds of secondary explants on calli induction have been studied. Moreover, the critical concentration of G418 has been determined.Our results indicated that the propagation efficiency could be as high as 27 000 times. The whole progress of from bulb scale to new plant formation only need 9 months. The medium supplement with 2.0 mg/L 2,4-D in conjunction with short basal stem segment as secondary explant was most favorable for embryogenic calli formation and the critical concentration of G418 was 50 mg/L.关键词
东方百合/两步外植体/胚性愈伤Key words
Oriental Hybrid lily/ Two-step explant/ Embryogenic callus分类
农业科技引用本文复制引用
张静,刘菊华,徐碧玉,贾彩红,张建斌,谭光兰,金志强..东方百合高效离体再生体系的建立[J].热带作物学报,2011,32(2):269-272,4.基金项目
中央级科研院所基本科研业务费项目(No.ITBB-YB071). （No.ITBB-YB071）
