热带作物学报2011，Vol.32Issue(2)：278-282,5.
一株拮抗香蕉枯萎病菌的链霉菌分离和鉴定
Isolation and Identification of a Streptomyces Strain against Banana Wilt Disease Caused by Fusarium oxysporum f. sp. cubense
摘要
Abstract
A Streptomyces strain named HW1 against banana wilt caused by Fusarium oxysporum f. sp. cubense race 4(FOC4) was isolated from a hot spring in Hainan Province. The morphological characteristics, cultural characteristics, physiological, biochemical characteristics and 16S rDNA sequences analysis of Streptomyces HW1 were studied. Besides, the co-culture tests in soil between HW1 and FOC4 were studied at different temperature.The results showed that, on PDA medium, the substrate mycelia of HW1 had no partition, and the spores were round and clustered on the aerial mycelia. The clone of HW1 was white when it started growing on PDA medium,after matured, the aerial mycelia of HW1 became gray gradually and finally became brown because of spores.Besides, HW1 could produce yellow ,soluble pigment on PI)A medium. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related species. HW1 strain was preliminarily classed to Streptomyces costaricanus. The co-culture tests in soil between HW1 and FOC4 indicated that HWI showed better resistance on FOC4 when grown at 45 C than at room temperature.关键词
香蕉枯萎病/链霉菌HW1/分离/鉴定/共培养Key words
Banana wilt disease/ Streptomyces HW1/ Isolation/ Identification/ Co-culture分类
生物科学引用本文复制引用
卢娟,夏启玉,孙建波,卢雪华,王宇光,张昕..一株拮抗香蕉枯萎病菌的链霉菌分离和鉴定[J].热带作物学报,2011,32(2):278-282,5.基金项目
中央级公益性科研院所基本科研业务费专项(No.ITBBZX2008-2-3). （No.ITBBZX2008-2-3）
