热带作物学报2011，Vol.32Issue(2)：289-292,4.
野苘蒿变叶病病原物的分子鉴定
Molecular Identification of the Pathogeny of Gynura crepidioides Phyllody Disease in Hainan
摘要
Abstract
A PCR product (about 1.4kb) was amplified by employing phytoplasma universal 16S rRNA gene primers (R16mF2/R16mR1)and total DNA sample extracted from diseased Gynura crepidioides phyllody as the amplified template. Then the amplified fragments were cloned and sequenced. The results of sequences comparison of Blast program in GenBank, construction of phylogenetic tree and analysis of iPhyClassifier indicated that the phytoplasma caused Gynura crepidioides phyllody disease in Hainan belonged to Peanut witches-broom phytoplasma A subgroup(16Sr Ⅱ-A subgroup). This is the first report of phytoplasma in naturally infected Gynura crepidioides.关键词
野茼蒿变叶病/植原体/16S/rDNA/分子鉴定Key words
Gynura crepidioides phyllody disease/ Phytoplasma/ 16S rDNA/ Molecular identification分类
农业科技引用本文复制引用
车海彦,郑文虎,符瑞益,温衍生,罗大全..野苘蒿变叶病病原物的分子鉴定[J].热带作物学报,2011,32(2):289-292,4.基金项目
海南省自然科学基金项目(No.310069) （No.310069）
中央级科研院所基本科研业务费专项(No.2011hzs1J007). （No.2011hzs1J007）
