中国肺癌杂志2011，Vol.14Issue(6)：507-511,5.DOI:10.3779/j.issn.1009-3419.2011.06.06
广西地区非小细胞肺癌中K-ras基因点突变的研究
Study on Point Mutations of K-ras Gene in Non-small Cell Lung Cancer in Guangxi
摘要
Abstract
Background and objective Recent studies indicated that non-small celllung cancer (NSCLC) patients with mutant K-ras were resistant to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKls).The aim of this study is to explore the relationship between the mutation of K-ras gene and NSCLC in Guangxi by detecting the point mutations in codon 12, l3 and 61 of K-ras gene in NSCLC.Methods The point mutations in codon 12, l3 and 61 of K-ras gene were detected by single-strand conformation polymorphism (SSCP) analysis of polymerase chain reaction (PCR) products and DNA sequencing analysis in 105 cases of NSCLC tissues and 30 cases of adjacent normal tissues.Results No point mutation in codon 12, 13 and 61 of K-ras gene was found in 105 cases of NSCLC tissues and 30 cases of adjacent normal tissues.In this study, the mutation frequency of K-ras gene in NSCLC was O (0/105).Conclusion The high proportion of K-ras gene in wild-type indicates that patients with NSCLC in Guangxi could take more benefits from the therapy with EGFR-TKIs.关键词
肺肿瘤/K-ras基因/点突变Key words
Lung neoplasms/ K-ras gene/ Point mutation分类
医药卫生引用本文复制引用
钟炜祥,陈铭伍,冼磊,李满红..广西地区非小细胞肺癌中K-ras基因点突变的研究[J].中国肺癌杂志,2011,14(6):507-511,5.基金项目
本研究受广西科学研究与技术开发计划项目(No.10124001A-44)和广西研究生教育创新计划项目(No.2010105981002M181)资助 （No.10124001A-44）
