分析化学2011，Vol.39Issue(6)：815-820,6.DOI:10.3724/SP.J.1096.2011.00815
超高效液相色谱-串联质谱法测定草鱼肉中阿苯达唑及其代谢物残留
Determination of Albendzole and Its Metabolites in Grass Carp Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method of ultra performance liquid chromatography-tandem mass spectrometry (UPLCMS/MS) has been developed for the determination of albendzole(ABZ) and its metabolites, alben-dazole sulfoxide (ABZSO), albendazole sulfone (ABZSO2) and albendazole aminosulfone (ABZ-2-NH2SO2) in grass carp meat. The samples were extracted with alkaline ethyl acetate and cleaned up by n-hexane. Chromatographic separation was achieved by UPLC and the detection was performed by tandem mass spectrometry using deuterated isotope internal standard. The UPLC-MS/MS analysis was rapid and can be achieved in less than 4 min. In the range of 0.1-10 μg/kg, the analytes were in good linearity, and the correlation coefficients were 0. 9991-0. 9996. The recoveries of ABZ, ABZSO, ABZSO2 and ABZ-2-NH2SO2 spiked at levels of 0. 5-5. 0 μg/kg, were 98. 4％ - 102. 6％, 97.1％-103.4％, 98.8％-103.7％, 101. 1％-104.2％, the limit of quantity (LOQ) were 0.2, 0.1, 0.2, 0.1 μg/kg, respectively. The Intra-day RSD were less than 4.8％, and Inter-day RSD was less than 3.0％. The UHPLC-MS/MS method is rapid, sensitive, reproducible and suitable for the determination of ABZ and its metabolites关键词
超高效液相色谱-串联质谱/阿苯达唑/草鱼Key words
Ultra performance liquid chromatography tandem mass spectrometry/ Albendzole/ Grass carp引用本文复制引用
张小军,郑斌,张虹,余海霞,陈雪昌,郭远明,梅光明..超高效液相色谱-串联质谱法测定草鱼肉中阿苯达唑及其代谢物残留[J].分析化学,2011,39(6):815-820,6.基金项目
本文系浙江省水产加工产业创新团队项目(No.2011R09031-15)和浙江省重大科技专项项目(No.2008C02013)资助 （No.2011R09031-15）
