首页|期刊导航|中国神经再生研究（英文版）|Silencing gamma-aminobutyric acid A receptor alpha 1 subunit expression and outward potassium current in developing cortical neurons
中国神经再生研究（英文版）2011，Vol.6Issue(18)：1404-1409,6.DOI:10.3969/j.issn.1673-5374.2011.18.008
Silencing gamma-aminobutyric acid A receptor alpha 1 subunit expression and outward potassium current in developing cortical neurons
Silencing gamma-aminobutyric acid A receptor alpha 1 subunit expression and outward potassium current in developing cortical neurons
摘要
Abstract
We used RNA interference (RNAi) to disrupt synthesis of the cortical neuronal γ-aminobutyric acid A receptor (GABAAR) α1 in rats during development, and measured outward K+ currents during neuronal electrical activity using whole-cell patch-clamp techniques. Three pairs of small interfering RNA (siRNA) for GABAAR α1 subunit were designed using OligoEngine RNAi software. This siRNA was found to effectively inhibited GABAAR α1 mRNA expression in cortical neuronal culture in vitro, but did not significantly affect neuronal survival. Outward K+ currents were decreased, indicating that GABAAR α1 subunits in developing neurons participate in neuronal function by regulating outward K+ current.关键词
γ-aminobutyric acid A receptor/α1 subunit/neuron/development/outward potassium currentKey words
γ-aminobutyric acid A receptor/α1 subunit/neuron/development/outward potassium current引用本文复制引用
Tao Bo,Jiang Li,Jian Li,Xingfang Li,Kaihui Xing..Silencing gamma-aminobutyric acid A receptor alpha 1 subunit expression and outward potassium current in developing cortical neurons[J].中国神经再生研究（英文版）,2011,6(18):1404-1409,6.基金项目
This study was supported by the National Natural Science Foundation for Youth (Inhibitory neurotransmitter receptor changes in nerve cells after developing brain injury),No.30400483. （Inhibitory neurotransmitter receptor changes in nerve cells after developing brain injury）
