南方农业学报2011，Vol.42Issue(4)：358-363,6.
广西桑树种质资源亲缘关系的SRAP分析
Assessing the phylogenetic relationship amongst Guangxi mulberry (Morus atropurpurea Roxb.) germplasm resources using SRAP markers
摘要
Abstract
[Objective]The present experiment was conducted to study the phylogenetic relationship amongst Guangxi mulberry (Morus atropurpurea Roxb.)germplasm resources using SRAP (sequence-related amplified polymorphism) markers. [ Method ]The polymorphism amongst 28 Guangxi local mulberry germplasm resources, belonging to Guangdong mulberry family, was assessed using SRAP molecular marker technique and their genetic diversity was analyzed using cluster analysis. [ Result]The results showed the amplification of 144 bands in 28 mulberry germplasm using 22 pairs of SRAP primer combinations. Of which, 45 bands (31.81％) showed polymorphism. On an average, the number of total and polymorphic bands/primer pair primer combination was recorded as 6.55 and 2.05, respectively. The genetic similarity coefficients among mulberry resources ranged from 0.8264 to 1.0000, with an average of 0.8944. UPGMA results showed 3 clear clusters for the 28 mulberry materials in dendrogram. The first group included 20 local mulberry materials, the second included 7 self-breeding mulberry materials and the third had only one material. [Conclusion ]High genetic variations amongst Guangxi mulberry germplasm resources were observed, and the genetic diversity and phylogenetic relationship of local mulberry germplasm were found in accordance with their habitat. The results of our experiment will be helpful in classification and breeding of the mulberry genotypes.关键词
桑树/种质资源/遗传多样性/SRAP/聚类分析Key words
mulberry(Morus atropurpurea Roxb.)/ genetic diversity/ SRAP/ clustering analysis分类
农业科技引用本文复制引用
林强,宾荣佩,朱方容,邱长玉,陈小青,赵卫国,李乙,曾燕蓉,朱光书,唐燕梅..广西桑树种质资源亲缘关系的SRAP分析[J].南方农业学报,2011,42(4):358-363,6.基金项目
现代农业产业技术体系建设专项项目(nycytx-27-gw105) （nycytx-27-gw105）
广西科学研究与技术开发计划项目(桂科攻0992012,桂科攻10100010-4) （桂科攻0992012,桂科攻10100010-4）
广西自然科学基金项目(桂科自0991177) （桂科自0991177）
