南方农业学报2011，Vol.42Issue(4)：364-367,4.
广西野生大豆种质资源SSR引物筛选
Selection of SSR primers for studying genetic diversity amongst Guangxi wild soybean (Glycine soja) germplasm
摘要
Abstract
[Objective]The present study was aimed to screen molecular marker primers showing high polymorphism in order to study genetic diversity amongst Guangxi wild soybean (Glycine soja) gennplasm. [Method]The genetic diversity amongst 22 Guangxi wild soybean germplasm materials, collected at different periods, was analyzed by using 60 pairs of SSR primers. [Result]The results indicated that the allelic variation of 60 SSR primers ranged from 1.0 to 8.0 with an average of 3.5/primer. Twenty-three SSR primers showed better polymorphism with allelic variation above 4. A distinct allelic variation of SSR primers in different linkage groups was observed which ranged from 1.67 to 5.00. Amongst all the linkage groups, group J showed the least allelic variation (1.67), while it was the highest (5.00) in B2 and H groups. [Conclusion] The screened polymorphic SSR primers were suitable for studying genetic diversity of Guangxi wild soybean.关键词
野生大豆/SSR引物/筛选/等位变异Key words
wild soybean (Glycine soja)/ SSR primer/ selection/ allelic variation分类
农业科技引用本文复制引用
曾维英,陈渊,梁江,汤复跃,韦清源,王典结..广西野生大豆种质资源SSR引物筛选[J].南方农业学报,2011,42(4):364-367,4.基金项目
国家大豆产业体系南宁大豆综合试验站资助项目(nycytx-004) （nycytx-004）
广西青年科学基金项目(桂科青099171) （桂科青099171）
广西农业科学院基本科研业务费专项项目[200814(基)] （基）
广西农业科学院科技发展基金项目(2007005) （2007005）
