应用化学2011，Vol.28Issue(4)：458-463,6.DOI:10.3724/SP.J.1095.2011.00326
光谱法研究有机农药污染物与DNA的相互作用
Spectrometric Study on the Interactions Between Organic Pesticide Pollutants and DNA
摘要
Abstract
The interactions of organic pesticide pollutants including, dichlorvos ( DDVP) , omethoate (OME) , dimethoate(DIM) and plenazine-1-carboxylic acid (PC A) , with the telomere DNA were investigated with ultraviolet spectroscopy, fluorescence spectroscopy and circular dichroism ( CD). The results indicated that the organic pesticide pollutants interact with DNA mainly through the intercalative mode. The apparent binding constants of organic pesticides with DNA were calculated to be 1. 17× 106, 1.48 × 106, 4. 52 ×105, and 1. 80 × 106 L/mol, respectively. The apparent binding constants revealed that the order of the relative binding affinity is PCA ＞ DIM ＞ DDVP ＞ OME. The CD measurement indicated that these organic pesticide pollutants caused instability of the structure of the duplex DNA by weakening the it-it stacking of DNA base pairs. To some extent, it also affected the secondary structure of DNA, leading to the relaxing of the twin helix structure.关键词
有机农药污染物/脱氧核糖核酸/相互作用/嵌入模式Key words
organic pesticide pollutants, deoxyribonucleic acid, interaction, intercalative mode分类
化学化工引用本文复制引用
卜晓阳,吴敏,周家宏,杨小弟,陆天虹,李卉卉..光谱法研究有机农药污染物与DNA的相互作用[J].应用化学,2011,28(4):458-463,6.基金项目
国家自然科学基金(20902048,20875047)、江苏省高校自然科学基金(09KJB150004)、江苏省科技重大项目-江苏省生物医药材料测试服务平台开放基金(BM2007132)、生命分析化学教育部重点实验室开放基金(KLACLS07006)资助项目 （20902048,20875047）
