大豆科学2011，Vol.30Issue(2)：303-305,3.
大豆子叶节植株再生体系的研究
Research on Cotyledonary Nodes Regeneration System of Soybean
摘要
Abstract
The adventive shoots were induced from cotyledon nodes with soybean cultivar Liaodou 17, Liaodou 18 and Liaodou 23 as explants. With the increase of 6-BA concentration, the number of adventitious shoots on each explant showed an increasing trend, when O. 05 mg · L-1 IBA and 4.0 mg · L-1 6-BA were added into 1/2MSB medium, the induction rate of adventive shoots is the highest. Compared the effect of explants, the explants of reserving half cotyledon was better than the cotyledon nodes without cotyledon. Therefore, the optimum culture medium for elongation of adventitious bud was 1/2MSB + 0.5 mg · L-1 6-BA + O. 05 mg · L-1 IBA, and the optimum culture medium for rooting was 1/2MSB + 1.0 mg · L-1 IBA.关键词
大豆/子叶节/不定芽/植株再生Key words
Soybean/ Cotyledonary node/ Adventive shoots/ Plant Regeneration分类
农业科技引用本文复制引用
赖冰冰,韩阳,李春风,张兴政,张立军,宋书宏..大豆子叶节植株再生体系的研究[J].大豆科学,2011,30(2):303-305,3.基金项目
国家科技攻关资助项目(2003ba538c). （2003ba538c）
