北京中医药大学学报2011，Vol.34Issue(5)：333-336,4.
都梁滴丸提取物的含量测定
Quantitative determination of extract from Duliang Gutta Pills
周爱德 1周玉新 2雷海民1
作者信息
- 1. 北京中医药大学中药学院,北京,100102
- 2. 河南大学药学院
- 折叠
摘要
Abstract
Objective To establish a method for quantitative determination of extract from Duliang Gutta Pills by HPLC. Methods The HPLC procedure was performed on the chromatographic column of Kromasil C18(4.6 mm ×250 mm, 5 μm). The mobile phase for ferulic acid was CH3CN-H20 (1 mL of H3PO4 in 1 L of water, 17: 83 ) with the detection wavelength being 316 nm. The mobile phase for imperatorin and isoimperatorin was CH3CN-H20 (70: 30) with the detection wavelength being 300 nm. Results Ferulic acid showed a good linear relationship at a range from 0. 023 4 to 0. 140 6 μg ( r =0. 999 9) and the average recovery was 100.4％ ( RSD = 0.39％ ). Imperatorin showed a good linear relationship at a range from 0. 016 1 to 0. 322 0 μg, (r =0. 999 9) and the average recovery was 102. 9％ ( RSD =0.65％ ). Isoimperatorin showed a good linear relationship at a range from 0. 004 4 to 0.087 2 μg (r=0.999 9) and the average recovery was 100.0％ (RSD =0.24％ ). Conclusion The method was simple, sensitive and accurate, which can be used for the quality control of extract from Duliang Gutta Pills.关键词
HPLC/都梁滴丸/阿魏酸/欧前胡素/异欧前胡素Key words
HPLC/ Duliang Gutta Pills/ ferulic acid/ imperatorin/ isoimperatorin分类
医药卫生引用本文复制引用
周爱德,周玉新,雷海民..都梁滴丸提取物的含量测定[J].北京中医药大学学报,2011,34(5):333-336,4.
