大豆科学2011，Vol.30Issue(2)：219-223,5.
安徽省大豆根瘤菌表型多样性研究
Phenotypic Diversity of Soybean Rhizobia in Anhui Province
摘要
Abstract
Thirty-two unknown strains were obtained after isolation and purification from soybean nodules which collected in different areas of Anhui province. One hundred and twelve physiological and biochemical indexes of their nutrient utilization, antibiotic sensitivity, stress tolerance and other traits were tested. The results showed that there were much differences in carbon utilization, antibiotic resistance and stress tolerance among the strains from different areas and even the same area. Fiftyseven traits of all tested characteristics had differences among strains. 93.8％ strains could grow in the medium containing 3.0％ NaC1 and 78.1％ strains grow in the 7.0％ NaCI medium. None of the strains could grow under the condition of initial pH3, but all except AH09 and AH24 could grow at initial pH12. All the strains could survive after treated at 4℃ for 24 h, but all died at 60℃ for 24 h. All strains could grow in the medium containing 300 mg· L-1 gibberellin or fosfomyein, but sensitive to streptomycin. Cluster analysis showed that soybean rhizobia of Anhui province were divided into two groups, 29 strains belonged to one group, and had high similarity.关键词
大豆/根瘤菌/表型多样性Key words
Soybean/ Rhizobia/ Phenotypic diversity分类
农业科技引用本文复制引用
吴萍,何庆元,李正鹏,史均,祝嫦巍,盛伟..安徽省大豆根瘤菌表型多样性研究[J].大豆科学,2011,30(2):219-223,5.基金项目
安徽科技学院生物学重点建设学科资助项目(AKXK2010 2-1) （AKXK2010 2-1）
安徽省教育厅自然科学基金资助项目(KJ2011Z066). （KJ2011Z066）
