广西科学2011,Vol.18Issue(2):164-168,5.
毕赤酵母发酵甘露醇产乙醇的条件研究
Study on Conditions of Ethanol Fermentation of Manni- tol by Pichia angophorae
摘要
Abstract
Ethanol fermentation of different concentration of mannitol by Pichia angophorae was carried out in 250ml Erlenmeyer flasks. The optimum composition of fermentation medium was determined by the tests with concentration of 20g/L,40g/L, 60g/L and 80g/L of mannitol.Then an orthogonal test was designed and performed, searching for the best fermentation condition. The results indicated that the optimum medium composition for ethanol fermentation was 20g/L mannitol, 0. 3g/L yeast extract, 0. 3g/L malt extract, 5g/L (NH4)2SO4,2g/L KH2PO4 and 0.4g/L MgSO4 · 7H2O. Besides, the optimal fermentation condition was 32℃,150rpm, initial pH value 4.5 and 150ml fermentation liquor with a yield of 0.45 g ethanol(g mannitol)-1关键词
毕赤酵母/甘露醇/发酵/乙醇Key words
Pichia an gophorae/ mannitol/ fermentation/ ethanol分类
化学化工引用本文复制引用
潘诗翰,陈姗姗,黄庶冰,龙明华,黄庶识..毕赤酵母发酵甘露醇产乙醇的条件研究[J].广西科学,2011,18(2):164-168,5.基金项目
广西自然科学基金重点项目(2010GXNSFD013029),广西科学院基本科研业务费项目(10YJ25WL01),人力资源和社会保障部留学人员科技活动项目择优经费项目(人社厅函[2010]412号)资助. (2010GXNSFD013029)