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毕赤酵母发酵甘露醇产乙醇的条件研究

潘诗翰 陈姗姗 黄庶冰 龙明华 黄庶识

广西科学2011,Vol.18Issue(2):164-168,5.
广西科学2011,Vol.18Issue(2):164-168,5.

毕赤酵母发酵甘露醇产乙醇的条件研究

Study on Conditions of Ethanol Fermentation of Manni- tol by Pichia angophorae

潘诗翰 1陈姗姗 2黄庶冰 1龙明华 3黄庶识2

作者信息

  • 1. 广西科学院,广西南宁,530007
  • 2. 广西大学,广西南宁,530004
  • 3. 广西师范大学生命科学院,广西桂林,541004
  • 折叠

摘要

Abstract

Ethanol fermentation of different concentration of mannitol by Pichia angophorae was carried out in 250ml Erlenmeyer flasks. The optimum composition of fermentation medium was determined by the tests with concentration of 20g/L,40g/L, 60g/L and 80g/L of mannitol.Then an orthogonal test was designed and performed, searching for the best fermentation condition. The results indicated that the optimum medium composition for ethanol fermentation was 20g/L mannitol, 0. 3g/L yeast extract, 0. 3g/L malt extract, 5g/L (NH4)2SO4,2g/L KH2PO4 and 0.4g/L MgSO4 · 7H2O. Besides, the optimal fermentation condition was 32℃,150rpm, initial pH value 4.5 and 150ml fermentation liquor with a yield of 0.45 g ethanol(g mannitol)-1

关键词

毕赤酵母/甘露醇/发酵/乙醇

Key words

Pichia an gophorae/ mannitol/ fermentation/ ethanol

分类

化学化工

引用本文复制引用

潘诗翰,陈姗姗,黄庶冰,龙明华,黄庶识..毕赤酵母发酵甘露醇产乙醇的条件研究[J].广西科学,2011,18(2):164-168,5.

基金项目

广西自然科学基金重点项目(2010GXNSFD013029),广西科学院基本科研业务费项目(10YJ25WL01),人力资源和社会保障部留学人员科技活动项目择优经费项目(人社厅函[2010]412号)资助. (2010GXNSFD013029)

广西科学

OACSTPCD

1005-9164

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