分析化学2011，Vol.39Issue(6)：925-933,9.DOI:10.3724/SP.J.1096.2011.00925
核酸适体技术在食品安全分析中的应用
Application of Aptamers in Food Safety
摘要
Abstract
Aptamers are all new ligands with a high affinity for considerably differing molecules ranging from largetargets as proteins over peptides, complex molecules to drugs and organic small molecules or even metal ions. These molecules are identified and selected through an in vitro process called systematic evolution of ligands by exponential enrichment (SELEX). Aptamers are widely used, including medical and pharmaceutical basic research, drug development, diagnosis, and therapy. Analytical and separation tools bearing aptamers as molecular recognition and binding elements are another big field of application. The SELEX method was modified over the years in different ways to become more efficient and less time consuming, to reach higher affinities of aptamers selected and higher automation of process. In reviewing this technology, the article covers the topic, following a general introduction, under the headings: the SELEX protocol, the development of aptamers by use of SELEX, and the application of aptamers in food safety.关键词
核酸适体/指数富集配体系统进化/食品安全/残留分析/有机小分子/评述Key words
Aptamers/ Systematic evolution of ligands by exponential enrichment/ Food safety/Residue/ Small organic molecules/ Review引用本文复制引用
徐敦明,吴敏,邹远,张强,吴崔晨,周昱,刘贤进..核酸适体技术在食品安全分析中的应用[J].分析化学,2011,39(6):925-933,9.基金项目
本文系厦门市科技计划项目(No.3502Z20092008) （No.3502Z20092008）
福建省自然科学基金项目(No.2008J0107) （No.2008J0107）
国家质检总局科技计划项目(No.20101K150) （No.20101K150）
国家重点基础研究发展计划(973)项目(No.2010CB732400)资助. （973）
