中国全科医学2011，Vol.14Issue(24)：2765-2767,3.
补体C3mRNA在大鼠脑出血模型中的表达及眼镜蛇毒因子对其表达的影响
Expression of C3mRNA in a Rat Model of Acute Experimental Intracerebral Hemorrhage and the Impact of Cobra Venom Factor
郑晓梅 1刘亮 2李小刚 3高毅滨 1袁云华1
作者信息
- 1. 646000四川省泸州市,泸州市人民医院神经内科
- 2. 泸州医学院附属医院神经外科
- 3. 泸州医学院附属医院神经内科
- 折叠
摘要
Abstract
Objective To investigate the mechanisims of complement C3 during the acute phase of intracerebral hemorrhage ( CH ) in rats and its role in the increasing of brain water content; to investigate the effects of cobra venom factor ( CVF )on C3mRNA expression and brain water content after intracerebral hemorrhage in rats. Methods Rat CH model was established by injecting autologous blood into the caudate nucleus of rats stereotaxically. SD rats were divided into three groups ( hemorrhagic group, sham operation group and CVF treatment group ) and sacrificed 3 h, 6 h, 12 h, 1 d, 3 d, 5 d, and 7 d after establishment of the model. Brain water content ( BWC ) was determined using wet and dry weight measurement of and mRNA levels of C3 were determined by RT - PCR method. Results Expression of C3mRNA increased 6 hours after CH and peaked 3 days after CH.Brain water content around the hematoma increased 3 h after CH and peaked 3 days after CH ( P ＜ 0.05 ), which decreased to normal levels at 7 d time. Positive correlations existed hetween brain water content and C3mRNA expression ( r = 0.758, P ＜0.05 ). C3mRNA expression around hematoma decreased significantly after CVF intervention in comparison with the hemorrhage group ( P ＜ 0.05 ). Brain water content in the intervention group was significantly lower than in the CH group ( P ＜ 0.05 ). Conclusion The complement cascade may be activated after CH with the expression of C3mRNA increased significantly, CVF intervention, which reduces C3mRNA expression and brain edema, exhibits a protection effect on the damaged brain tissue after CH.关键词
脑出血/补体C3/脑含水量/眼镜蛇毒因子Key words
Cerebral hemorrhage/ Complement C3/ Brain water content/ Cobra venom factor分类
医药卫生引用本文复制引用
郑晓梅,刘亮,李小刚,高毅滨,袁云华..补体C3mRNA在大鼠脑出血模型中的表达及眼镜蛇毒因子对其表达的影响[J].中国全科医学,2011,14(24):2765-2767,3.
