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不同利用方式对红壤坡地微生物多样性和硝化势的影响

张慧 袁红朝 朱亦君 陈春兰 谭周进 秦红灵

生态学杂志2011,Vol.30Issue(6):1169-1176,8.
生态学杂志2011,Vol.30Issue(6):1169-1176,8.

不同利用方式对红壤坡地微生物多样性和硝化势的影响

Microbial diversity and nitrification potential of sloping land red soil under different land use patterns

张慧 1袁红朝 2朱亦君 2陈春兰 2谭周进 2秦红灵3

作者信息

  • 1. 湖南农业大学生物安全科技学院,长沙,410128
  • 2. 中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,长沙,410125
  • 3. 湖南中医药大学基础医学院,长沙,410208
  • 折叠

摘要

Abstract

Soil microbial community plays a crucial role in ecological sustainability in response to different land use patterns. In this paper, soil samples were collected from the cropland, natural ly restored forestland, and tea garden on the red soil sloping land at the Taoyuan Agro-ecosystem Research Station (110·72″E, 28°52″N) under Chinese Academy of Sciences to study the effects of different land use patterns on the soil microbial diversity and nitrification potential. T-RFLP technique was adopted to study the diversity of bacteria, ammonia-oxidizing bacteria ( AOB) , and ammonia-oxidizing archaea ( AOA) , and aerobic culture method was used to study the nitrification potential. Under the three land use patterns, the diversity index of soil bacteria, AOB, and AOA showed the same variation trend, i. e. , cropland = tea garden > naturally restored for est land ( P<0. 05 ) . RDA analysis showed that the variations of soil physical and chemical properties under different land use patterns determined the community structure of soil AOB and AOA. Cropland soil had the highest nitrification potential, followed by tea garden soil, and naturally restored forestland soil. There was a significant positive correlation between the diversity index of soil bacteria, AOB , and AOA and the soil potential nitrification rate , especially for AOA, suggesting that AOA might play an important role in red soil nitrification.

关键词

细菌/古菌/氨氧化细菌(AOB)/氨氧化古菌(AOA)/T-RFLP

Key words

bacteria/ archaea/ ammonia-oxidizing bacteria ( AOB) / ammonia-oxidizing archaea ( AOA) / T-RFLP.

分类

生物科学

引用本文复制引用

张慧,袁红朝,朱亦君,陈春兰,谭周进,秦红灵..不同利用方式对红壤坡地微生物多样性和硝化势的影响[J].生态学杂志,2011,30(6):1169-1176,8.

基金项目

国家自然科学基金项目(40801098)资助. (40801098)

生态学杂志

OA北大核心CSCDCSTPCD

1000-4890

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