中国畜牧兽医2011，Vol.38Issue(4)：84-87,4.
定量检测牛奶中蛋白质ELISA方法的建立
Development of an Optimized ELISA for the Quantitative Detection of Protein in the Milk
摘要
Abstract
This experiment is aimed to develop a ELISA method to detect the precise content of protein in the milk specificly. Immune the rabbit with pure casein,the polyclonal antibodies against casein were obtained. The best working concentration of antigen and antibody was determined using the checkerboard test, and the reaction conditions of each steps were optimized.The 1 ％ BSA was served as blocking agent, and its incubation time was 120 min. The optimal dilution and incubation time of serum sample and enzyme-labeled second antibody were 1: 12000 for 90 min and 1 ∶ 3000 for 30 min respectively. The detection range of casein was 0. 063 to 0. 500 μg/mL. The method had good specificity, accuracy, repeatability, the range of regression rate was 97.6％ and 123％. The ELISA method for detecting the protein content of milk was successfully established.关键词
乳蛋白/酪蛋白/ELISA/定量检测Key words
milk protein/ casein/ ELISA/ quantitative detection分类
化学化工引用本文复制引用
李萌,许会会,高晋渝,雷连成..定量检测牛奶中蛋白质ELISA方法的建立[J].中国畜牧兽医,2011,38(4):84-87,4.基金项目
"十一五"国家科技支撑计划重点项目"国家重点领域认证认可推进工程"(2008BAK42B05). （2008BAK42B05）
