中国畜牧兽医2011，Vol.38Issue(4)：91-94,4.
人IL-3、GM-CSF和Hly基因的cDNA克隆及测序
Cloning and Sequencing of Human Genes cDNA of the IL-3、GM-CSF and Lysozyme
摘要
Abstract
The coding region of human genomic genes of the IL-3,GM-CSF and lysozyme (Hly) were obtained by PCR method. The IL-3 and GM-CSF genes were linked with the pMD19-T vector, and the Hly gene was linked with the pEASY-Blunt vector. Then the eukaryotes expression vector for these genes were constructed by insert these gene fragments into pIRESneo vector. Following, transfection the PK15 cells with these eukaryotes expression vector respectively. The total RNA of the transfected cells were extracted after 48 hours and the cDNA of the human genes of the IL-3,GM-CSF and Hly were obtained by RT-PCR method. The results showed that the sequences were completely homologous with the registered ones of the NCBI.关键词
IL-3基因/GM-CSF基因/Hly基因/cDNA/克隆/测序Key words
IL-3 gene/ GM-CSF gene / Hly gene / cDNA / cloning / sequencing分类
生物科学引用本文复制引用
孙英民,孙朕,樊宝良..人IL-3、GM-CSF和Hly基因的cDNA克隆及测序[J].中国畜牧兽医,2011,38(4):91-94,4.基金项目
农业部转基因重大专项"高品质转基因奶牛新品种培育"(2008ZX08007-001) （2008ZX08007-001）
农业部转基因重大专项"环境友好的转基因猪新品系培育"(2008ZX08006004) （2008ZX08006004）
国家863计划重点项目"家禽与昆虫蛹生物反应器研究"(2007AA100504). （2007AA100504）
