中国畜牧兽医2011，Vol.38Issue(4)：105-108,4.
牛轮状病毒TaqMan实时荧光RT-PCR快速检测方法的建立
Detection of Bovine Rotavirus by TaqMan Based Real-time Reverse Transcription Polymerase Chain Reaction Assay
摘要
Abstract
A TaqMan based real-time RT-PCR was developed for the specific detection of bovine rotavirus (BRV). The specific primers and probes were designed according to the VP6 sequences of BRV. It was found that the specificity of this assay was high without any cross-reactions and had no CSFV, BVD, MB and IBRV. A good linear correlation was demonstrated in the standard curve for the real-time PCR assay within the range of 102 to 109 copies, with a correlation coefficient of 0. 997. The detection limit of the real-time RT-PCR assay was 100 copies of viral RNA, indicating a good sensitivity of the assay. This TaqMan based real-time RT-PCR assay reported here is a valuable method with high specificity and sensitivity detection of BRV.关键词
牛轮状病毒/TaqMan实时荧光定量PCR/检测Key words
BRV/ TaqMan based real-time RT-PCR/ detection分类
生物科学引用本文复制引用
范晴,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤,谢丽基,彭宜..牛轮状病毒TaqMan实时荧光RT-PCR快速检测方法的建立[J].中国畜牧兽医,2011,38(4):105-108,4.基金项目
国家百千万人才工程人选专项资金资助(945200603),广西科技攻关与新产品试制项目(桂科合0992033-5). （945200603）
