中国畜牧兽医2011，Vol.38Issue(4)：113-117,5.
蛇四棘食道口线虫线粒体cox1基因的克隆及序列分析
Cloning and Sequence Analysis of Mitochondrial Cytochrome c Oxidase Subunit 1of Oesophagostomum quadrispinulatum Isolates from Changsha City
摘要
Abstract
To analyze the differences in cytochrome c oxidase subunit 1 (cox1) gene of Oesophagostomum quadrispinulatum isolated from Changsha city. The partial cox1 (pcox1) was amplified from each O. quadrispinulatum sample, and pcox1 sequences were aligned using the Clustal X 1.83. Sequence homology analysis was performed using the MegAlign program of the software DNAStar version 5.0 by comparing with the corresponding sequence of O. quadrispinulatum available in GenBankTM.The results showed that the lengths of pcox1 were 393 bp. Sequence comparison revealed that the similarity in pcox1 sequences among Changsha isolates and that of the O. quadrispinulatum available in GenBankTMwere over 98％. There was no significant variation in pcox1 sequences within O. quadrispinulatum, while inter-species difference was obviously. The results provided a foundation for further studies of molecular identification and molecular genetics of O. quadrispinulatum.关键词
四棘食道口线虫/cox1基因/序列分析Key words
Oesophagostomum quadrispinulatum / cox1 gene/sequence analysis分类
生物科学引用本文复制引用
陈文承,徐平源,李芬,刘伟,刘毅..蛇四棘食道口线虫线粒体cox1基因的克隆及序列分析[J].中国畜牧兽医,2011,38(4):113-117,5.基金项目
国家自然科学基金(30771616) （30771616）
湖南省科技计划一般项目(K0902144-21) （K0902144-21）
长沙科技计划一般项目(2010FJ3006). （2010FJ3006）
