中国畜牧兽医2011，Vol.38Issue(4)：124-126,3.
ELISA一步法快速检测牛奶中三聚氰胺的研究
Study on One-step ELISA Method for Detection of Melamine in Milk
摘要
Abstract
An one-step indirect competitive ELISA system for detection of melamine residues in milk was developed based on monoclonal antibody,and the performance about the detection system such as recoveries,sensitivity, precision and specification were evaluated. The random samples after detected by ELISA had confirmed by LC-MS/MS to access its practicality. The results showed that the calibration curve of this ELISA detection system had the linear detection of 10 to 810 ng/mL, the sensitivity of 10 ng/mL, and the recoveries of 84.0％ to 90.0％ in milk,with the cross-reactivity within 0. 1％. Therefore,the onestep ELISA established in this study is a practical method for determination of melamine residue in milk.关键词
三聚氰胺/牛奶/ELISA/一步法Key words
melamine/ milk / ELISA / one-step method分类
农业科技引用本文复制引用
赵宁,徐飞,吴小平,王战辉,李杰超,朱景慧,潘净茹..ELISA一步法快速检测牛奶中三聚氰胺的研究[J].中国畜牧兽医,2011,38(4):124-126,3.基金项目
农业科技成果转化资金项目(2010GB2A000009). （2010GB2A000009）
